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- Construct 'CONS00000609'
Construct
ID
CONS00000609
Name
PCi-Rora -658/-539 Fox mutated pFog::LacZ
Min Promoter
Reporter
Comment
this contruct is named Mutated Rora_AS2 enhancer in the paper. This construct contains the RORa regulatory region from -658bp to -539bp, in which the 5 Fox binding sites are mutated (TGTTT -> TGCTT).
Primers used: Rora_AS2enhancer_Fwd:5-ACGTTGTTTGCTCAGAAGAGCCG-3
Rora_AS2enhancer_Rev:5-CAAGCAAAACATTATCTTTCGCGTC-3
Show sequence
Length: 119
ACGTTGCTTGCTCAGAAGAGCCGAAGCAAGCATTACGGTGTCGTCGTGCTTGCATTTGGC
GTGCTTAATTGATAGCGCCATTCGCGAGTCTAAAGACGCGAAAGATAATGCTTTGCTTG