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CONSTRUCT

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  1. Construct 'CONS00001191' > 
  2. Construct 'CONS00000056' > 
  3. Construct 'CONS00001653' > 
  4. Construct 'CONS00000652' > 
  5. Construct 'CONS00000672'

Construct

ID

CONS00000672

Name

pCi-Pitx -2751/-2696 repeated x2/150bp spacer:CES2:LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)-150-(ab) in the paper. The region named D1ab in the paper was repeat once, and between the two fragments there is a spacer region of 150bp. This region can t drive expression in the neurohypophysis primordium.
The spacer region added was represents by "N".

This construct is testing Regulatory Region

REG00000703: Ci-Pitx -2751/-2696 repeated x2/150bp spacer

Sequence

Show sequence

Length: 260

AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNAAACGCGACGACCTCCACGGATTAAGGTAATATTT
CTTTTTATGTTGCCGCCCAG

Reference(s)

Show in situ data