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CONSTRUCT

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  1. Construct 'CONS00000370'

Construct

ID

CONS00000370

Name

pfog -290/1 otx a-element added::NLS-lacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct (amEts-pfog-290 construct in the paper) contains the entire Ci-fog cis regulatory region from -290bp to the ATG. The otx neural a-element was inserted upstream of pfog. Electroporation of this construct led to strong expression in neural precursors and weak sporadic expression in uninduced animal cells. The nucleotide +1 taken in reference corresponds to the ATG.

This construct is testing Regulatory Region

REG00000383: Ci-fog -290/1 otx a-element added

Sequence

Show sequence

Length: 351

CGTTATCTCTAACAGAAGTTTTCGAAAAAGAAATTGTTCAATATCTAAGATAGGAAGGCT
CAAAATTCAGGAAACGGTCCAAGACCGAAGCTCCAAAGCTCTTGTGTTCAGTTAAACTCT
GATAGTGAATAAGCTTCGTGTATTGTACCGGCCCATTGTCAATCATGCAAACTTGATATT
ATATTGACAAGAGAAGAAGGCAGTTTAAATTAAAACTCTAAAGTAGAGAGACATTAATCT
CAGCTGACAAGGCAGGTGGTCACAGTAAGTTCATTTAAATAGTTGGCCAACAATAGCCTT
TCCAAGAAAGTATTTTTGTTCCAGGTCTATACAAAAATAACACACATAACC

Reference(s)