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- Construct 'CONS00000369'
Construct
ID
CONS00000369
Name
pfog -290/1 bra enhancer added::NLS-lacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct (ebra290 construct in the paper) contains the entire Ci-fog cis regulatory region from -290bp to the ATG. The Ci-bra enhancer covering position -470 to -62 was inserted upstream of pfog. Electroporation of this construct led to robust notochord expression. The nucleotide +1 taken in reference corresponds to the ATG.
Show sequence
Length: 409
ACTTACGGCAAATTGTAGTTAAAATTCATAATTACAAAACAATAAAGATCATATAAACAA
AATATTAAAGATCATATTAACAATATAATATAGATCATATTAATAGCGACAAACCTTATC
TGGTGTTACGTCACAATACAAACAAAATATTTCGACATGTCAATCAAAATCGGAAACCAA
GTTTCAACTTCCCACGCAAGACAATGGGAAAGTAACACGTCACAATACACTTGGTGACGT
CATATCACTAAAACAAACACAAGGTGTTCGATCCAGCTGTGAAAGTAAACATAGAGCGCC
ACCACACGAGCAACCCTCACAGCTGGATGCCACCACCTACGGCGCACTTTCAACAAACAT
AAAATTTCAAAAAGAAGAAGTTATGACGTCACAATCCTGTATAAACTTG