- Construct 'CONS00000112'
Construct
ID
CONS00000112
Name
pBra -71/17 first codons::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
This construct contains the Ci-Bra cis regulatory region from -71bp to the first 17 codons. This construct lacks the 3 E-box sequences. Electroporation of this construct induces no expression : staining in the notochord and ectopic expression in the muscles and mesenchyme which is detected when the E-box sequences are present, is absent. The nucleotide +1 taken in reference corresponds to the first codon (contrary to the article where it corresponds to the TATAA box).