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CONSTRUCT

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  1. Construct 'CONS00000112'

Construct

ID

CONS00000112

Name

pBra -71/17 first codons::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This construct contains the Ci-Bra cis regulatory region from -71bp to the first 17 codons. This construct lacks the 3 E-box sequences. Electroporation of this construct induces no expression : staining in the notochord and ectopic expression in the muscles and mesenchyme which is detected when the E-box sequences are present, is absent. The nucleotide +1 taken in reference corresponds to the first codon (contrary to the article where it corresponds to the TATAA box).

This construct is testing Regulatory Region

REG00000119: pBra

Reference(s)