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CONSTRUCT

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  1. Construct 'CONS00000111'

Construct

ID

CONS00000111

Name

pBra -143/17 first codons::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This construct contains the Ci-Bra cis regulatory region from -143bp to the first 17 codons. This construct lacks 2 of the 3 E-box sequences. Electroporation of this construct induces no expression in the notochord and there is a severe reduction in the ectopic expression in the muscles and mesenchyme. The nucleotide +1 taken in reference corresponds to the first codon (contrary to the article where it corresponds to the TATAA box).

This construct is testing Regulatory Region

REG00000118: Ci-Bra -143/17 first codons

Reference(s)