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CONSTRUCT

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  1. Construct 'CONS00000865'
  2. Construct 'CONS00001664'
  3. Construct 'CONS00000112'
  4. Construct 'CONS00000634'
  5. Construct 'CONS00000109'

Construct

ID

CONS00000109

Name

pBra -299/17 first codons delta 2Su(H)::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This construct (named -250bp w/o Su(H) in the publication) contains the Ci-Bra cis regulatory region from -299bp to the first 17 codons in which the two Su(H) DNA binding motifs are deleted. Electroporation of this construct induces the loss of staining in the notochord and ectopic expression in the tail muscle and the mesenchyme.The nucleotide +1 taken in reference corresponds to the first codon (contrary to the article where it corresponds to the TATAA box).

This construct is testing Regulatory Region

REG00000116: Ci-Bra -299/17 first codons delta 2Su(H)

Reference(s)