- Construct 'CONS00000107'
Construct
ID
CONS00000107
Name
pBra -300/17 first codons Su(H)1 mutated::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct contains the Ci-Bra cis regulatory region from -300bp to the first 17 codons in which a single nucleotide substitution was created in the Su(H)1 motif (TTCCCAC to TTgCCAC). This subtitution results in reduced binding of a GST/Ci-Su(H) fusion protein. The mutagenized transgene exhibits weak staining in the tail muscles and trunk mesenchyme, but is essentially inactive in the notochord. The nucleotide +1 taken in reference corresponds to the first codon.
