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CONSTRUCT

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  1. Clone 'AHC0AAA230YC07'
  2. Construct 'CONS00000781'
  3. Construct 'CONS00001620'
  4. Construct 'CONS00000057'
  5. Construct 'CONS00000382'

Construct

ID

CONS00000382

Name

pBra -324/17 first codons::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct (named -275bp in the publication) contains the Ci-Bra cis regulatory region from -324bp to the first 17 codons. Electroporation of this construct induces strong ectopic expression in tail muscle without interfering with notochord expression. The nucleotide +1 taken in reference corresponds to the first codon (contrary to the article where it corresponds to the TATAA box).

This construct is testing Regulatory Region

REG00000397: Ci-Bra -324/17 first codons

Reference(s)