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CONSTRUCT

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  1. Construct 'CONS00000096'

Construct

ID

CONS00000096

Name

pBra -483/17 first codons ZicLb2 mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains the Ci-Bra cis regulatory region from -483bp upstream the TSS and the 17 first codons which is mutated in the 5 ZicLb (call ZicLb2) DNA regulatory motifs, B2: CCAGCTGTG go to CgAcgTcTG . Mutation in ZicLb2 DNA Binding motif reduce reporter expression. The nucleotide +1 taken in reference corresponds to the first codon.

This construct is testing Regulatory Region

REG00000103: Ci-Bra -483/17 first codons ZicLb2 mutated

Reference(s)