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CONSTRUCT

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  1. Clone 'AHC0AAA224YD22' > 
  2. Construct 'CONS00000731' > 
  3. EST 'AHC0AAA231YJ14_RM1' > 
  4. Construct 'CONS00000092'

Construct

ID

CONS00000092

Name

pBra -483/17 first codons 2ZicL mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains the Ci-Bra cis regulatory region from -483bp upstream the TSS and the 17 first codons which is mutated in the two ZicLb DNA regulatory motifs, B1: CACAGCTGG (complementary sequence: CCAGCTGTG) go to CAgAcgTcG (CgAcgTcTG) and B2: CCAGCTGTG go to CgAcgTcTG. Mutation in the two ZicL DNA Binding motif abolishes reporter expression. The nucleotide +1 taken in reference corresponds to the first codon.

This construct is testing Regulatory Region

REG00000099: Ci-Bra -483/17 first codons 2ZicL mutated

Reference(s)

Show in situ data