- Molecular Tool 'Ci-Bra -3.5Kb/Ci-Su(H)DBD' >
- Transcript 'Moocul.CG.ELv1_2.S80...' >
- Transcript 'Phmamm.CG.MTP2014.S9...' >
- Construct 'CONS00000092'
Construct
ID
CONS00000092
Name
pBra -483/17 first codons 2ZicL mutated::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct contains the Ci-Bra cis regulatory region from -483bp upstream the TSS and the 17 first codons which is mutated in the two ZicLb DNA regulatory motifs, B1: CACAGCTGG (complementary sequence: CCAGCTGTG) go to CAgAcgTcG (CgAcgTcTG) and B2: CCAGCTGTG go to CgAcgTcTG. Mutation in the two ZicL DNA Binding motif abolishes reporter expression. The nucleotide +1 taken in reference corresponds to the first codon.