- Construct 'CONS00000091'
Construct
ID
CONS00000091
Name
pBra -483/17 first codons 2Su(H) mutated::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct contains the Ci-Bra cis regulatory region from -483bp upstream the TSS and the 17 first codons which is mutated for the two Su(H) binding site (Ci-Su(H)1 sequence including CCT-------ACG was change to CCT-------AAT and Ci-Su(H)2 including the sequence AAT-------GAA was change to AAT-------TAT). These alteration do not influence the binding of GST/Ci-Su(H) fusion protein in vivo but result in a slightly stronger staining in vivo. The nucleotide +1 taken in reference corresponds to the first codon.