- Construct 'CONS00000326' >
- Construct 'CONS00001469' >
- Construct 'CONS00000087'
Construct
ID
CONS00000087
Name
pBra -3500/first 17 codons ZicLb2 mutated::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct contains the entire Ci-Bra cis regulatory region from -3,5Kb the first 17 codons which is mutated in the ZicLb2 DNA regulatory motifs, B2: CCAGCTGTG go to CgAcgTcTG. Mutation in ZicLb2 DNA binding motif did not induce a significant expression level decrease of reporter in notochord cells compare to the same construct mutated in ZicLb1 DNA binding motif. The nucleotide +1 taken in reference corresponds to the first codon.