- Construct 'CONS00000086'
Construct
ID
CONS00000086
Name
pBra -3500/first 17 codons ZicLb1 mutated::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct contains the entire Ci-Bra cis regulatory region from -3,5Kb to the first 17 codons which is mutated in the ZicLb1 DNA regulatory motifs, B1: CACAGCTGG go to CAgAcgTcG. The transcription start site is the nucleotide +1. Mutation in ZicL-b1 DNA binding site reduces expression of the reporter in notochord cells. About 53% of stained embryos still exhibit the reporter expression in most notochord cells. The nucleotide +1 taken in reference corresponds to the first codon.