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CONSTRUCT

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  1. Construct 'CONS00001098'
  2. Construct 'CONS00000134'
  3. Construct 'CONS00000086'

Construct

ID

CONS00000086

Name

pBra -3500/first 17 codons ZicLb1 mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains the entire Ci-Bra cis regulatory region from -3,5Kb to the first 17 codons which is mutated in the ZicLb1 DNA regulatory motifs, B1: CACAGCTGG go to CAgAcgTcG. The transcription start site is the nucleotide +1. Mutation in ZicL-b1 DNA binding site reduces expression of the reporter in notochord cells. About 53% of stained embryos still exhibit the reporter expression in most notochord cells. The nucleotide +1 taken in reference corresponds to the first codon.

This construct is testing Regulatory Region

REG00000094: Ci-Bra -3500/17 first codons ZicLb1 mutated

Reference(s)