- Construct 'CONS00000085'
Construct
ID
CONS00000085
Name
pBra -3500/first 17 codons 2ZicL mutated::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct contains the entire Ci-Bra cis regulatory region from -3,5Kb to the first 17 codons which is mutated in the two ZicLb DNA regulatory motifs, B1: CACAGCTGG (complementary sequence: CCAGCTGTG) go to CAgacgTcG (CgAcgTcTG) and B2: CCAGCTGTG go to CgAcgTcTG . Mutation in both ZicL DNA-binding motifs abolished or reduce reporter expression. The nucleotide +1 taken in reference corresponds to the first codon (contrary to the article where it corresponds to the TATAA box).
