- Construct 'CONS00000016'
Construct
ID
CONS00000016
Name
pZicL -658/codon7 Fox2/4/5 mutated::LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
construct named Fox2/4/5-mut in the paper. This construct includes the 658bp of the 5 flanking sequence of the Ci-ZicL promoter and the 7 first codons with mutations on the putative Fox2, Fox4 and Fox5 binding sites :
-Fox2 AATTGTTTGTGT -> AATTGGGCGTGT
-Fox4 AGGCAAATATCG -> AGGCGGGTATCG
-Fox5 AAATAAATAAAG -> AAATGGGTAAAG
The expression of lacZ in A6.2 and A6.4 was diminished but not in B6.2 and B6.4. The expression was not completely abolished even in A-line cells
