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CONSTRUCT

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  1. Construct 'CONS00000076'

Construct

ID

CONS00000076

Name

pMLC2 -438/205 44bp mutated::GFP

Min Promoter

Endogenous Promoter

Reporter

GFP

Comment

643-bp construct containing the -438bp of 5 flanking sequence, the first exon, the first intron, and a 5 portion of the second exon of Ci-MLC2, connected with an enhanced GFP coding sequence and which the two motifs M2 and the 18-bp sequence between them had been mutated (construct named Ci-MLC2-EGFP (deltaM2p/d) in the paper). The expression in tail muscles is lost

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : -502bp/+141bp

This construct is testing Regulatory Region

REG00000085: Ci-MLC2 -438/205 44bp mutated

Reference(s)