- Construct 'CONS00001366'
Construct
ID
CONS00001366
Name
p.Cirobu.REG.KH2012.C5:4471851-4472163>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord. Sequence of the primers used : Forward : TtctagaTAGTGACTCAAGTTTCAAAGTTATGTTAAG Reverse : AggatccACAGCATAGTCACAACGGCTTTC Lowercase letters correspond to XbaI/BamHI restriction site.
Show sequence
Length: 313
TAGTGACTCAAGTTTCAAAGTTATGTTAAGCTTTAAGAAAAGTGTAGACAGGCGTACTGC
TTAACAAAACATTTGCAGACTATTAAAAATTATTTAAAGTATTAATAAGCGCTACTTTAA
TATTTTTGTATGTTTACCAGTAATGAGTTTCGTACAGAATACGTTGCGCCGTGAATTGAA
ATGTGTGTTAACTTAACCTAGATTGAATGCATTCCGTCTCGAGTACAAATGCCTTTCTGA
TTTGTTGTCTTGTTTGTTTTGCGTCTCTCTCGCTCTTGTTCAATATTTAGAAAGCCGTTG
TGACTATGCTGTG