- Construct 'CONS00001374'
Construct
ID
CONS00001374
Name
p.Cirobu.REG.KH2012.C12:712628-713987>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord. Sequence of the primers used : Forward : AtctagaAGAAATGTTTAATATTGCCCAAGTCCC Reverse : TggatccTCCGGACGACGCCATTCC Lowercase letters correspond to XbaI/BamHI restriction site.
REG00001354: Cirobu.REG.KH2012.C12:712628-713987|CbpD,CbpE,CbpN
Show sequence
Length: 1,360
AGAAATGTTTAATATTGCCCAAGTCCCCTTGACGTCACAAATAATTAATTTTCTTCTCTA
TGTGACGTTGAGTCTGTTTGTTTACCTCAGATAGGTTCGAACCCGGTTAACCCTTAGCCT
TCAACCTCATTTACCAACACGTGACAGATAAATTAGTTTGTTGTCACCTGCACGGGTTTT
GAACCTTAAACCCCACGCCTCTTGCGATAGCATTCAAGCATCTGCGGACTCGAAATTCTA
GGCATCTCTTCAATGTTAAGAGTGACACTGACACGCTATGTTGTTATATAAACTAGGGAA
GTGTTTTGTATTTGCCGGGTAAGCGTGGAATGCGGCACACCCCGTAACCCCGAGCAAGCA
ACACGTATTTCCATCCATTCGTGACCAACCCTCCACACTTAACCATCGTATATGTTTGAA
CGTTTATCTTCACCCAAACCCAACTTACCTACACTGAGGTTTGTTTGATGTATTTGCTGG
CGTTTGCGCAATGCTACTACAGCATTATCTTATGTAGCAATGCTGATAGTTTCGTATAAC
TTATTATAATCGCTCCAAAAAGGTTGCAGCATTTCACACCATACTTTATGCCACACTGTA
ATAGTTTAATCGAATACAACTTAAATATGACTTTTGTTACTTACGAAGAAATGCTTGAAT
AGATCGTGACAACGCGAATCTGTATTATATTATCTCCATTCGTCGTTATAGCTACGGGGG
AATTTTTTCCCCCCCTTTTCGTTATTTTTTTTTTTTAAAAAAAAAAAAATTGACGAAAGA
GCGACAGGAAATAACGACAAACGACATTCTATTTTGGTGTCGCCTAAAAATAGCGTGTGT
GTAAAGAACGGCCTTTACCGAATATAAACCGCACATTTAGTCGCGTGTGTATTTTATAAA
ATATCCTGAATATTTTTGTGATGGGCTAAAAGTCACGTGCTTATATTCTATTGTATGTTG
TGCCACGTCACAATACAGTAAGTGATTTGTTATCGATGAGGGGTTGTGTGAATTCCGTGC
CACTCCCAACTTGGATGACAAGGGGATTAATCACGGAAAGCTGCAACTTCCACCTGGTAA
TCACGTTTTCACTACGTGCACATTCATTTATTCCACAACCAAAAAAAAAAATCAAAAAAG
AGAAAACAGACTTTTTATTACCCACAAAGTTACATATACGTTGTAATTCGTAAGCAGGCA
TGACGTGTACGAAACCGAACATTCGTGCTATAAAGACTGTCGTTGGCCTGCCACGCGAGG
ATAAAACAAGTTACAATCTTCCCTTCACCGCTAACGGTTAAATAGTGGTGTATGACGTCA
CATAGGACGTCCCGCGGGAGTTGGAATGGCGTCGTCCGGA