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CONSTRUCT

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  1. Construct 'CONS00000074'

Construct

ID

CONS00000074

Name

pMsxb 130bp/238bp N3 added/mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains two tandem repeats of the mutated N3 motif sequence atcgtcgcggccgaagcgTGAccaagtcgt -> atcgtcgcggccgaagcgCAGccaagtcgt in 5 of the +130bp to the +238bp of the 5 flanking Ci-msxb enhancer sequence. Expression is observed only in the head mesenchyme. Expression is lost in the sensory vesicle and in the neck/visceral ganglion.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : 119bp/227bp

This construct is testing Regulatory Region

REG00000084: Ci-msxb 130/238 N3 motif added/mutated

Reference(s)