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CONSTRUCT

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  1. Construct 'CONS00000073'

Construct

ID

CONS00000073

Name

pMsxb 130bp/238bp N3 added::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct named N3-0.1 in the paper was prepared by fusing two N3 (atcgtcgcggccgaagcgtgaccaagtcgt) tandem repeats upstream the +130bp to the +238bp of the 5 flanking Ci-msxb enhancer sequence. Expression is observed in the sensory vesicle, in the neck and in the visceral ganglion.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : 119bp/227bp

This construct is testing Regulatory Region

REG00000083: Ci-msxb 130/238 N3 motif added

Reference(s)