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CONSTRUCT

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  1. Construct 'CONS00000072'

Construct

ID

CONS00000072

Name

pMsxb -117/238 N3 added/mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains two tandem repeats of the mutated N3 motif sequence (atcgtcgcggccgaagcgTGAccaagtcgt -> atcgtcgcggccgaagcgCAGccaagtcgt) in 5 of the -117bp to the +238bp of the 5 flanking Ci-msxb enhancer sequence. Expression is observed in mesodermal territories but expression in the nervous system is lost.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : -129bp/227bp

This construct is testing Regulatory Region

REG00000082: Ci-msxb -117/238 N3 motif added/mutated

Reference(s)