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CONSTRUCT

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  1. Construct 'CONS00000071'

Construct

ID

CONS00000071

Name

pMsxb -117/238 N3 added::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct named N3-0.35 in the paper was prepared by fusing two N3 (atcgtcgcggccgaagcgtgaccaagtcgt) tandem repeats upstream the -117bp to the +238bp of the 5 flanking Ci-msxb enhancer sequence. Expression is observed in the precursor of otolith and ocellus,in the neural fold and in the mesoderm cells.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : -129bp/227bp

This construct is testing Regulatory Region

REG00000081: Ci-msxb -117/238 N3 motif added

Reference(s)