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CONSTRUCT

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  1. Construct 'CONS00000151'

Construct

ID

CONS00000151

Name

p29h10 -903/-350::LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

this construct contains the -903bp to -350bp of 5 flanking region of 29h10 gene. It was cloned into a reporter gene containing the minimal Ci-Fkh promoter region and lacZ. Expression is restricted to the endoderm and TVCs as seen for the endogenous gene but this enhancer also drives expression in the tail muscle, although this is not seen in the endogenous expression pattern.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : -900bp/-400bp

This construct is testing Regulatory Region

REG00000159: 29h10 -903/-350

Reference(s)