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CONSTRUCT

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  1. Construct 'CONS00000129'

Construct

ID

CONS00000129

Name

pTitf1 -553/39 M20 mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains a LacZ transgene with the region from -553bp of the 5prime flanking Cititf1 enhancer sequence which contains mutations by transversion of the nucleotides in position -403bp to -399bp (TgaTT to TccTT) (construct M20 in the paper). This electroporation showed a decrease of the endodermal expression.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates of this region in the paper : -355bp/+238bp

This construct is testing Regulatory Region

REG00000136: Cititf1 -553/39 M20 mutated

Reference(s)