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CONSTRUCT

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  1. Construct 'CONS00000048'

Construct

ID

CONS00000048

Name

pCi-Sna -650/87 E7/E6/E5 mutated::NLS-LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This construct includes the sequence from -650 to +87bp of the Sna gene in which the all AC-core E-Boxes (E7, E6, E5) are mutated into a -> TT-core E-box. Intense staining is seen in the neural tube (arrows). Some embryos also exhibits expression in the secondary muscle and the sensory vesicle. While expression in primary muscles is abolished, some embryos continue to show staining in trunk mesenchyme.

This construct is testing Regulatory Region

REG00000061: Ci-Sna -650/87 E7/E6/E5 mutated

Reference(s)