- EST 'AHC0AAA228YG20_RM1' >
- Construct 'CONS00000042' >
- Construct 'CONS00000043' >
- Construct 'CONS00000059' >
- Construct 'CONS00000055'
Construct
ID
CONS00000055
Name
pCi-Sna -672/-168 pfkh Anti::NLS-LacZ
Min Promoter
Reporter
Comment
This construct includes the sequences from -672bp to -168bp of the Ci-Sna 5 regulatory region attached to the heterologous Ciona forkhead promoter region. In this construct the Ci-Sna 5 DNA fragment is placed in the opposite (Anti) orientation as the direction of Ci-fkh/LacZ transcription. This construct enhancer directs expression in the primary muscles and mesenchyme but not in the TVCs. Note that the same region placed in front of the p-bra minimal promoter has no activity, highlighting the importance of basal promoter choice.