- Construct 'CONS00001324'
Construct
ID
CONS00001324
Name
p.Cirobu.REG.KH2012.C8:2621713-2622953_(Cirobu.REG.KH2012.C9:4425113-4425279)x4>GFP
Min Promoter
Reporter
Comment
This construct tests the effect of the putative repressor sequence of Wnntun5 enhancer on FoxD.b enhancer. Four repeats of Wnttun5 repressor were inserted into the enhancer of FoxD.b, and the chimeric sequence was ligated to the Brachyury basal promoter upstream of GFP reporter gene. The insertion of the four repeats is directly downstream of the Tcf7 binding site cluster, as it is located in WT Wnttun5 enhancer.
Show sequence
Length: 1,909
TTTGTTTACTTTTTTTACTTTGTTTATGTTAAGTAAAGTTTAATTTCATTAAAGATGTTA
GTTCGACTTTAATCGTAGCTTAGTTTGACTGATGTCGCGAGTGATTAGGCGATAAGAAGG
CGAGAAATATCATCGATGTTTGTTCCCGATATTTGGCGGCGTTTGTGGCGTTTGAAAGAG
GCGAGTTCGAGTCGCGAGTCTTATTTCAAGGCCGATCTCCGCATACGGGGATGCGCAAGC
GACTGATCTATTTGATAATTGGCGGTTTCCTCGCCCCTAGTGCTGGGCAGGGGTTGGAAT
CGAGCAATTACGATTGCGATCGCCGCCGACCACCTATTGGGAGGCCGAGACTCGTCCGGG
TATATTATTATTTCAAGGCCGATCTCCGCATACGGGGATGCGCAAGCGACTGATCTATTT
GATAATTGGCGGTTTCCTCGCCCCTAGTGCTGGGCAGGGGTTGGAATCGAGCAATTACGA
TTGCGATCGCCGCCGACCACCTATTGGGAGGCCGAGACTCGTCCGGGTATATTATTATTT
CAAGGCCGATCTCCGCATACGGGGATGCGCAAGCGACTGATCTATTTGATAATTGGCGGT
TTCCTCGCCCCTAGTGCTGGGCAGGGGTTGGAATCGAGCAATTACGATTGCGATCGCCGC
CGACCACCTATTGGGAGGCCGAGACTCGTCCGGGTATATTATTATTTCAAGGCCGATCTC
CGCATACGGGGATGCGCAAGCGACTGATCTATTTGATAATTGGCGGTTTCCTCGCCCCTA
GTGCTGGGCAGGGGTTGGAATCGAGCAATTACGATTGCGATCGCCGCCGACCACCTATTG
GGAGGCCGAGACTCGTCCGGGTATATTATCGATCTTTTCATCTTTATCTCGGTTGAATTG
ATTTGTCGTGAATAAATTGGGAAGTTGTGTCGATTGGAACATCAGTTGCAAGAATGAGTT
GAACAGTTGGTCCAATTCAAACGTTACTTGCAGTAAAACTTGTGTGGACAAGTTATAACT
TAATACGAGGTGTATATAAAGTCGTTAGAATTTTACGAATCAGTTGACAAATATAAATAA
AGGTACAGGTAGTTTTGTTGAACATTGCGACAGTCTGACTGTGTTTTCAGTTTCGCTAAA
TTTCTCGTACGGTAAAATTGTTTACTAAAAAATAAGGGCGTACGTAAATCAATTCTGGGT
TGGGTAGCTTTAAGCATTACAATAACGTACATATTGCCTGTTTACAAATATAGCAGTTAT
ATTTAAATATATACTAAGAATAAAGGTGCAGCTCATTCAAATTTAATCGCTTTACCGATT
CTCTCAATTAAGAATAGGAGAAAACGCGTATTTAGATAACATTTAACGGGAAACGACTAC
AGTCCTAATATTGGAACTACAGACGTTCAATAAACTCGTTAGCTTGACATTTATTTCAAT
ACTGTTTGTGTTACGTCACGGGTATTTTTGAGTCATTATTTTAACGATTGGTCGGATTAT
TTGAAAACCTTTACCACAGTGTGTTGTTAGAAAATAGACGCCATTTTTACCAGCGGAACC
AGCATTTTGCCAAATTCAAATTTAATAATTTTTTTCAGTTGTTTTCAACATTTTGGGACA
CTTCTCAAAAAAGCGAAATGTTTTTTATGAAACTCAGTTTTTAAAAACAGCGAAAACACG
CATATTTTTGAACGCAACCATTAATCGATTAAGTTGGACGTTTTTCTTGTAAATGATAAA
TTAATTAGCCTTCTTCTTTTGCTTAGAAAACATTGTAATATAATCTTTTGAAAAATCTGT
TGCTGATCAACGGAATTATAAACGAGATCTAGGATTCGAAGAAAAAAGCGGTTGCTGGGA
GAATTGAAGAAGTGGATTTGAATAAATTGTCGCGTTTTGCAAAGTGCTC
