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- Construct 'CONS00001326'
Construct
ID
CONS00001326
Name
p.Cirobu.REG.KH2012.L65:310840-311303>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the notochord. Sequence of the primers used : Forward : AtctagaCGACTTACCCCAACCTACTATATTC Reverse : AggatccGATTACAGAACCGAATCGTGTTCG Lowercase letters correspond to XbaI/BamHI restriction site.
Show sequence
Length: 464
CGACTTACCCCAACCTACTATATTCTACAGTTTACCTTTTGCTTGAAACGTTAAGTTTTA
TTTACCAAAATAACACCAGGCGTGTACGCCGCCATGTGACTCTAAATATAACACCTACAG
ATGTTATATTTACACCGGTTTGTTAAATAGGTTGCGAAGCGAACTTACAAACGCATGTAA
GGCGTTGGTGTCGAGGCTGTTAAATTGAGATGTACCAGACACACACGAGATATCTATCCC
CTATCTTATCACAGTAAGAGCAGAACAGCGTTCAACCTAACACACAGGACGCGTAACATC
ACAATAGGACATTCCCAACGCTGTTTAACACTGTATTGACAACAAGAGAAATCTAATACG
AACCCACGTATACAAAACACATCTTTTTACCGCTCTGACAATGCTCTTTATGACCTCATG
TCATTATTGTCCCCTTCAAACGAACACGATTCGGTTCTGTAATC