Aniseed - Construct

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Construct 'CONS00001331'

Construct

ID

CONS00001331

Name

p.Cirobu.REG.KH2012.L65:311070-311303_BraMut3>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives strong LacZ expression in the notochord.

This construct is testing Regulatory Region

REG00001311: Cirobu.REG.KH2012.L65:311070-311303_BraMut3|Dyrk2/3/4

Sequence

Show sequence

Length: 234

CGACTTACCCCAACCTACTATATTCTACAGTTTACCTTTTGCTTGAAACGTTAAGTTTTA
TTTACCAAAATAACACCAGGCGTGTACGCCGCCATGTGACTCTAAATATAACACAGCAAG
ATGTTATATTTACACCGGTTTGTTAAATAGGTTGCGAAGCGAACTTACAAACGCATGTAA
GGCGTTGGTGTCGAGGCTGTTAAATTGAGATGTACCAGACACACACGAGATATC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data