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- Construct 'CONS00001340'
Construct
ID
CONS00001340
Name
p.Cirobu.REG.KH2012.C9:35651-35811>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord. Sequence of the primers used : Forward : TGGCtctagaGGGGACATATATTGAATTAC Reverse : CAATccatggCAGCATGTCTAGCTTTTGTGC Lowercase letters correspond to XbaI/NcoI restriction site.
Show sequence
Length: 161
GGGGACATATATTGAATTACAATATATTTAGATTAGGAATACATTACTGGGTCAGTGAAG
CAAACGCTGCAACTTATCCACCAATTGCTTTCCAGCATTGAGCAAATGTTGAAGTCACGT
GTTACTTTTCACGCCTTATTGCACAAAAGCTAGACATGCTG