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- Construct 'CONS00001341'
Construct
ID
CONS00001341
Name
p.Cirobu.REG.KH2012.C9:35651-35739>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord, central nervous system, mesenchyme and tail muscles. Sequence of the primers used : Forward : AGctcgagCTTATCCACCAATTGCTTTCCA Reverse : GAtctagaCAGCATGTCTAGCTTTTG Lowercase letters correspond to XhoI/XbaI restriction site.
CTTATCCACCAATTGCTTTCCAGCATTGAGCAAATGTTGAAGTCACGTGTTACTTTTCAC
GCCTTATTGCACAAAAGCTAGACATGCTG