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CONSTRUCT

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  1. Construct 'CONS00000821' > 
  2. Construct 'CONS00001263' > 
  3. Construct 'CONS00001346'

Construct

ID

CONS00001346

Name

p.Cirobu.REG.KH2012.C10:2815687-2816162>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord.

This construct is testing Regulatory Region

REG00001326: Cirobu.REG.KH2012.C10:2815687-2816162|Ubp4,Ubp8,Ubp11

Sequence

Show sequence

Length: 476

AGGACCTCACCCACAAGAATAAATAAAAACCCACTAAGAATAAATAAAAACAATAGTACC
GAACTTTATATTCTTGCTAGAAATGTGTTTTGGCATTCACGAACATACAAATATTTTTGA
TTGTCGCACGGTTTTCGTCCATTTTACATACCTCAAACAACTGCCAAAAAATACGCCCAA
AAAATAATCACCCACAAAGTAACATACATGGTAACTCGTAAGCTGGCACGAGGTGTTAAA
CCCATGTTATGACGACTGTCGTTTTCCGGCCACGCGAGGATAAAGTTACATTCATTCATT
CATTTACCTCTTCCTACAACATACTATTCCACACAACTTTTACAAAACACTTGATAATTT
GAATAAAGTTTAATACTGTAACCAGATGATCAGAGATTGGAGAAAAAAAGCAAGAGGTCG
AGGTTAATTTGCAAAAAGTCTTCACAACACAATATCACAGAAGTTCCTACACAGTA

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data