Aniseed - Construct

Construct

ID

CONS00001349

Name

p.Cirobu.REG.KH2012.C10:2816165-2816946>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord.

This construct is testing Regulatory Region

REG00001329: Cirobu.REG.KH2012.C10:2816165-2816946|Ubp4,Ubp8,Ubp11

Sequence

Show sequence

Length: 782

TTTCATTGATCCAGTTAATGTGGAAGATATATCACGCTGGTTATAGGAAAACCAATCAAG
GCCAGTTTGCATCTGTTTTAACGGTTCGCAAAAACCAGCATTTTCTAGGTATAGTAGGGT
GGGGGAAGATGGGACACCTTTTCGTCCCATTTGGTAATAAACAAAGAACATTCAAGGATT
TATTAAACCGTATCCCTACGACTTTCATAGACTGTCGTTAATTGTTTAAAACACGATCAG
GATATTTGGATATTAAGTGCTAAAGGTGTCCCATCTTCCCCCACAGTACTCTATATTAGC
TCGTCAACTGCTTACTTAAACGTCAACTGCTTACTTAAACTTACTTGAACTAAACAGCAC
TGTGGTGACTAGACACACGGCGAAAACTAACTAGGCTCCAACAGGCTGTCAAACAGTTTC
GTTGCGCAAGAAAAAAGCGCTGCCTCTAAAACGAGACCAGCGGCACGAAAAGGCGGGGAT
ACTGAAATAATAATTACAACGAATCATAAAGCGCTTGTGCTGAAATTAAAATTGTCATGC
TTTTCATACTGAAATTTCCAAATTGTAATATTTAAAATGCTTTTCAGTATAAATGTTAAT
AATATAGTAAGGTGGGAAAAGATGGGATACCTTCAGCACATAATATCCAAATACCTTGAT
CGTGGTTTATTAAACAACTAACAACGGTAAATAGAAGGAATGTCGCGGCACCGCTCGCAT
GAAAGACTTGAATCGGCTTTCCGGGTATTGAGCAAAATCTGGCGTAAATCCCAAGTCCTT
GA

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data