Aniseed - Construct

Construct 'CONS00000220' >
Construct 'CONS00001391' >
Construct 'CONS00001353'

Construct

ID

CONS00001353

Name

p.Cirobu.REG.KH2012.C10:2816564-2816650>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord, central nervous system and tail muscles. Sequence of the primers used : Forward : TtctagaTTAGCTCGTCAACTGCTTACTTAAAC Reverse : TTccatggTCGCCGTGTGTCTAGTCACC Lowercase letters correspond to XbaI/NcoI restriction site.

This construct is testing Regulatory Region

REG00001333: Cirobu.REG.KH2012.C10:2816564-2816650|Ubp4,Ubp8,Ubp11

Sequence

Show sequence

Length: 87

TAGCTCGTCAACTGCTTACTTAAACGTCAACTGCTTACTTAAACTTACTTGAACTAAACA
GCACTGTGGTGACTAGACACACGGCGA

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data