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CONSTRUCT

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  1. Transcript 'Haaura.CG.MTP2014.S9...' > 
  2. Transcript 'KH2012:KH.C8.316.v1....' > 
  3. Construct 'CONS00001360'

Construct

ID

CONS00001360

Name

p.Cirobu.REG.KH2012.S345:68522-68746_Sp1mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in mesenchyme but not in notochord.

This construct is testing Regulatory Region

REG00001340: Cirobu.REG.KH2012.S345:68522-68746_Sp1mut|KH.S345.4

Sequence

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Length: 226

ACTAGCCTTAGGATAGGCAGGGCCAAGTGCCTGGCCATCCTTGCAAGAAGGGCCACGTAC
TAGATGGCCGCCCGAAGGCCACCTCAAGCCATTATCAAATGGCAAAGGCTCTAAAGAGTG
GGGGCTACCTACCGTAAGCTGCATGGCGCCGGGGCAAAAGCGCACCAAAACAAACTAGCC
GGTGGATAGGCCGGGCCTGCAAGAAGGGCCAGGTACTAGATGGCAC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data