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CONSTRUCT

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  1. Construct 'CONS00000731' > 
  2. EST 'AHC0AAA231YJ14_RM1' > 
  3. Construct 'CONS00000092' > 
  4. Construct 'CONS00000649' > 
  5. Construct 'CONS00001248'

Construct

ID

CONS00001248

Name

p.Cirobu.REG.KH2012.C9:5648755-5649375>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested sequence has no enhancer activity in the notochord.

This construct is testing Regulatory Region

REG00001245: Cirobu.REG.KH2012.C9:5648755-5649375|DDR1; DDR2; MUSK

Sequence

Show sequence

Length: 621

GTAATTTGAGTTGTAAGAAGGTGACCGATTCTTGACACGTTCCGGGGCCGGGTCGTCTTT
TTGCCGGGGAGCGTGCTCAAATTTTACGATCATTCTCGGAGTAGCTGAACGACGTCCGTG
AGGTAATTATAAGGCGGGGTCAATTCCACGAAAACGCACTGCTGGAGATACGGTGTTCAG
AGACGCATTGTTCAACGAGGGTTTACGACAGGCAGTTACAGTAAATTGCTGCTAATAATT
CGTTAAATAGTGCCAAGTAATTTTAGAAGATCTTCGGAATTAAAATGAAAATTATTTTCA
AATGATTCGATTGATTTGATAGTTTGATAACGCTAGAAGTTCGTCCCAATATGACCTTTC
CACATTACGATGTCGGTCCTTCAAAAGTATGTTGTGTTAACGGCGAGTGTACTACTTTCA
ATCGAGCAAATATTAGATACAAAAATGGGTCAGTACTTCAAACTTAAGGTTTCGCGATTG
CTGGCGCTCTCTGTTTGTGTAGTAGGGAATAAATTATAACCATTGGTGTTTGTAAACCGG
TCAAATGTTGTCTTGGTTCAACAAAACCTGGACAGTTTGATTGGTGTTAATTTATGAGCA
ACAAAATGCGCTCCCATGTTT

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data