Aniseed - Construct

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Construct 'CONS00001303'

Construct

ID

CONS00001303

Name

p.Cirobu.REG.KH2012.C7:3853970-3854222_Bra2mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the notochord despite the mutation of the second brachyury putative binding site. It is also active in head epidermis and mesenchyme.

This construct is testing Regulatory Region

REG00001284: Cirobu.REG.KH2012.C7:3853970-3854222_Bra2mut|Ephrin3

Sequence

Show sequence

Length: 253

CTCTACAGTATACGGAGAGTCCCCACCCATTAAACAAACGTAATATTAATTGTTAGATAG
ATATGATTCACACAGGATGTATTTGTGGTGTCTGGATTAACCCCCAGTAAGAATTCTCTG
CCATACAATAGCAGCGTGTTTGAATTACTGCTTGTTTTGGCAAGTTGATTAAGTCAGGTC
AATCTAGGTCAAGAACACACACACACTGTATGTATATACACAACACAGATGTAATATAAA
CATTGTAGTATTG

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data