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CONSTRUCT

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  1. Construct 'CONS00001433'

Construct

ID

CONS00001433

Name

p.Phmamm.REG.CG.MTP2014.S128:57500-58015>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in endoderm, TLCs, mesenchyme, notochord, TVCs, muscle, nerve cord and sensory vesicle territories

This construct is testing Regulatory Region

REG00001411: Phmamm.REG.CG.MTP2014.S128:57500-58015|Fgf9/16/20

Sequence

Show sequence

Length: 516

GGGCCACAGTGTCAAAATTATGGTTTTTGCGCATATGCATGTGGGGCATCGTTGGTGCCA
CGTATATTTTGATTGACAGCCGGTGCGCTTGACGAGGCGTCGTGCAGTGCTCGTGGGCGA
AAGTGCAATGTGGAGAATTCGCTAATATTTGCCGTTTAATCCCGAACGCATGCGGTCAGA
AGGCGATACACAGAGATTAAAAATCTAATGTATACAATGCAACAATAGCAGCGGGTGAGC
GAGAAAAAGGGCGAATCGTGCCCTATACCGACGAGGTCTTCGCCAGGTCTTTTAATGAGA
CGTCGGCCGCTGTGCAGAAAAAAGTTGACGAGTGGGCAGCTTGAAAAAGTGTGCGTTCAC
CGAAAGCGAAGTCGCTTCCTTTTATTATCGTGCCCCAATCATGGAAAGTCACAGATCAGT
CGTGAACGCTCATGGCGTTCCCAACTTCATGTGAAATCAATGGAATGATAATGAGAAATT
AGAAATGTATGTGTCATTCAGATCAGGTTGCACCAA

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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