Aniseed - Construct

Construct 'CONS00001442' >
Construct 'CONS00001443'

Construct

ID

CONS00001443

Name

p.Phmamm.REG.CG.MTP2014.S253:122003-122412>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in endoderm and TLCs.

This construct is testing Regulatory Region

REG00001421: Phmamm.REG.CG.MTP2014.S253:122003-122412|FoxD

Sequence

Show sequence

Length: 410

TGAGAAACTAGGACACCAAAAGCAGTGGGAAAAGGCTTAAAACACAATGTCCAATGTTCA
ATTCCAAGCGCGCCGTATGGTTAATAAGTTGCCGTTACAAGCCATTGAACAGTTGTTTCG
CATATTAGCGCTGTGTAGCGATGTTTCATGGCAAACAACGCCAGTCGAAGCGTTGAGCGT
CAAATCAGCCATAAATTGTCATTCCCTTTCTCGCAAACTCTCTCCGCAAAAGCCCGAAGT
CGAAGCTCCGAAAAGCAAACCCGACATTAGCGTTAGACGGCTCCGCGAGTCGGGTTTTTC
AAAGTAAGATGGCCGCGTCGCGCCAGGCTAAACTGTGGACGTTTTGAGGCGTTAAAGTAT
ACGTTTTTTTCTAAACATATTTAGTTGCAAAGTCTCAATATCATTTTTGG

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data