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CONSTRUCT

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  1. Clone 'ma218m13' > 
  2. Construct 'CONS00001450'

Construct

ID

CONS00001450

Name

p.Phmamm.REG.CG.MTP2014.S1711:8255-8854>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in endoderm, TLCs, mesenchyme, notochord and muscle.

This construct is testing Regulatory Region

REG00001428: Phmamm.REG.CG.MTP2014.S1711:8255-8854|Nodal

Sequence

Show sequence

Length: 600

CACTTAAGCTGAAGCGGACAAATATACCACCAGGTTTCACATCTGTTTTATTTAAAACAA
TTATGGGTCTGCCATGACATACGGTAACCTCATGTTACGGCTGTAGGTTCGTTGCAAGTC
GCTGGCTTTTCTGACGAACCAAGCTCTTTAATTCGGCCGCAAATGAGAGAAATGTAAACA
GTGACCCATAGACGCAACAAAAACTGAGGGCAAAATCAATGAGCGCTGGACCAATGGCAC
AGAGGCCTTACTGTGTTTACATTGTCACCTGCACGAACATCATCAAGGCTTGGCATACGG
GCAAATCGTTCTCTCTCTCCCTCTCTCTGGCCGAATCGCCAGGCGGCAAGATGCTAGAGG
AGAAAAAATGAACGCGGGTCCTGTTCCCATCCAGACTGCACGAGAGCTTCATCCCATTGT
GCGGAGAAAAAATGCCCGCCGGGACATCGTATAAATGGATCGAGAAAAGGTGACAAGTAT
CACATTCGCCCAACAGGTTTAAATTGAAACAGTCGGCGCAGTAGCATGATTTGTTATATG
GTGTTAATTTATTCAGAACAAATTAACAGGCGCAAAAGCCATAAACATGGAAAATGAAAA

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data