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CONSTRUCT

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  1. Gene 'Phmamm.CG.MTP2014.S3...' > 
  2. Construct 'CONS00001452'

Construct

ID

CONS00001452

Name

p.Phmamm.REG.CG.MTP2014.S1711:9716-10015>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in mesenchyme.

This construct is testing Regulatory Region

REG00001430: Phmamm.REG.CG.MTP2014.S1711:9716-10015|Nodal

Sequence

Show sequence

Length: 300

TCAAACTTAAAACGTAAACTTGCATTTTACTCTGACAACTTTTGGTAAAGTAATCACCTC
CGATGCACTAATTTATTTGCATAGAAAGCAGCACGCGTAGACACCGATAAGTTCAAACAC
CATCATCTGTCAGATGCAGTATTTGCACAAGCGACAAGAAAGTCACACTTCGGAAGGAAA
CCTGGATAAGCATTACCACAACCAAAATGTTTGATCGTTAAACTTTGTCTTATGTTCCGG
TTTATTTGAACTCAACGTGTTAAAGAAACCTTTGCACCTGTGTTCAGCTGGGTCGCTTTT

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data