Aniseed - Construct

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Construct 'CONS00000088' >
Construct 'CONS00001471' >
Construct 'CONS00001455'

Construct

ID

CONS00001455

Name

p.Phmamm.REG.CG.MTP2014.S166:50503-50840>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in the sensory vesicle.

This construct is testing Regulatory Region

REG00001433: Phmamm.REG.CG.MTP2014.S166:50503-50840|Otx

Sequence

Show sequence

Length: 337

ACCGTGGATTATCGCTTTCTAAAGTATTATGTTTATCCCTCTTTACTGTACACCACCCTT
TCACCAAGCCATCGCCGCACAAGCGTTGCGAACAGCACCGCGCTGAAATCGCGTCATCGC
CGGCAAACACATTGTGCGGCGGGGCGGGGTTGGTTGGGTCGGTTCGATCGCGAGAAGCAA
AATGGGCGAAAGCGTGATTAGCGGCGCTGCTCGAGTGTGGGAAAGCTGCACGACGCGTCT
TTATTAAAAAGTGCAGCCCCATGCAGCGATATCGGCTTATTATTTGGTCTACAACTTCGA
AGTTCCCACGTTGGGCATTTGTCCCTTTGTTCTTGGC

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data