Aniseed - Construct

Construct 'CONS00001061' >
Construct 'CONS00001435' >
Construct 'CONS00001457'

Construct

ID

CONS00001457

Name

p.Phmamm.REG.CG.MTP2014.S166:46706-46976>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in endoderm, mesenchyme, nerve cord and muscle.

This construct is testing Regulatory Region

REG00001435: Phmamm.REG.CG.MTP2014.S166:46706-46976|Otx

Sequence

Show sequence

Length: 271

TCACTGTGGGTTATTTGTAATTGGTCTTTAACAACAAGATGTAATTCGCAGTTAGTGATG
GCTCTCAGTGCGAAGGTAAACCTCCTTTGTCCCTCGCTTACCTGCTGTGTTTCCGCTGAT
ATGGACGTTGCTACATCCGTTCGCGCCACGATTCGCCCAGAACCTCTTCCATATCACCGA
AACACTGGCGTATCAGGTGCGAAGGGAAGTTTGGCCAAACTAAGTGATTGCACTTGCATT
TAGTTCGAAAACTTTCCCCTTCGATAAGGCA

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data