Aniseed - Construct

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Construct 'CONS00001482' >
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Construct 'CONS00001460'

Construct

ID

CONS00001460

Name

p.Phmamm.REG.CG.MTP2014.S160:16086-16385>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region is not able to drive LacZ expresion.

This construct is testing Regulatory Region

REG00001438: Phmamm.REG.CG.MTP2014.S160:16086-16385|Eph1

Sequence

Show sequence

Length: 300

AAAGGGGCAGTTTGTGGACGTGCGGCGCACTCCGCTTAATTCAGTGGCATTTCTCTGGAA
ACGCGCGCCACGAGGTTTGTAACTGCGCGTCTTTTGCAAAGGGAAGATTTGTTATTGTTT
GACGGTGTGTGGTTTACGTTGAAAGGAAATAGGCGGTCGTAAAGGGAAAGATAAGTGTTA
GGGCAGAAATAAATCAAAAAGAGGAAACAGTTTAATTGTGTGTATATGTTGCAAGCATGG
TAAATGTTTAAGTTTTTTACGTGGTGTGCTTGTTATTTGACATCTGTCGAGGACTCGAGG

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data