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CONSTRUCT

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  1. Region 'REG00001475' > 
  2. Construct 'CONS00001465'

Construct

ID

CONS00001465

Name

p.Phmamm.REG.CG.MTP2014.S597:19236-19686>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in endoderm.

This construct is testing Regulatory Region

REG00001443: Phmamm.REG.CG.MTP2014.S597:19236-19686|TTF1/Nkx2-1

Sequence

Show sequence

Length: 451

CAAACATCAAATTCCCGACATGAACGAAATTTGCCGACTTGCTAGATAAGAGTTTCCGTC
GGCAACGACAGCCGATGATCAACTTCCGATGTTTGTCACCTTTCATCATTCGCTTGTATG
TTTGCTTTCGGCCTCGAAACAAGTCGTCGTCACTAATTACAAGCGAGCCGCATCCACCGT
CAGCCGCAGACCATTTTCCGTCGATCCGATGACGTCACTTGGGCCGCTCAGTCATTTTTC
CCCTTCCCCTTTCGCGCGTCTACCTGGTCGCCTCGCACTGCATTCTAGCCTCCCTTTTTT
CGACGGGACGCCGCGACGGGACTCGGCCCGCCAGAAAACAGTGTCGCGGGTTGACAACAA
TGCGATGTCGCACAACAGAATGGTTCAGTTCAGGCAATGTTAGCGTGAGGAATATTGTCA
GGGACAGACAAGTGCAGCGAGCATTAGAAGT

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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