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CONSTRUCT

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  1. Construct 'CONS00001482' > 
  2. Construct 'CONS00001467'

Construct

ID

CONS00001467

Name

p.Phmamm.REG.CG.MTP2014.S597:17594-18238>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives weak LacZ expression in mesenchyme.

This construct is testing Regulatory Region

REG00001445: Phmamm.REG.CG.MTP2014.S597:17594-18238|TTF1/Nkx2-1

Sequence

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Length: 645

ACGCTGAACAATATCGGAATAGCTGACGATCGCGCGCAAATAACTGCGACAATTTGGGGG
CACGAAAACAACGCAGACGGCTCCCGAAGGCGGAACAAAGAAAAGTATTCGGACGACTAC
AAAAGCGCCTGTTCGAAATCGAGGCCCGGTGATTCGCCAAAACGGGTATGAAAACGGTCA
ACGAGGAAGCTCAGCTGCTCATGAGTGTCCGTTCGTCGCTTGCACACCGCGCTTCAAGCA
CTTAGAGGGTCCTTACGTTGCCTACGTTGAAGCCAGGGCGGACCACAACCGTGAGGCAAA
TTACGCAGGGGGGGGGGGACCGCGCTTCAAGCACTTAGAGGGTCCTTCTCAGCCTACGTT
GAAGCCAGGGCGGACCACAACCGTGAGGCAAATTACGCATCACATCGGCCCTGCTGCTGG
CAAAACCTAATGTTTTCAGATTGCGCCAAAATCGATAAACGCCGGTACTGCGAATGGAAA
AGTATTCGTTTTTTTTCTGGTGGTGACATCATGTTTTGATGTTTTCAGCGACGAGTCACA
GCTTTTCATGAAGCTTGTAGTTTGTCATGCGCGCGCATTTCGTTCAAAATCTGTATAAGC
GTTTTTCTGCTTGCCGCAAAATGGCGCTATGAGGCGAAAAGCTGT

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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