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CONSTRUCT

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  1. Construct 'CONS00001469'

Construct

ID

CONS00001469

Name

p.Phmamm.REG.CG.MTP2014.S597:19759-20374>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region is not able to drive LacZ expression.

This construct is testing Regulatory Region

REG00001447: Phmamm.REG.CG.MTP2014.S597:19759-20374|TTF1/Nkx2-1

Sequence

Show sequence

Length: 616

GTTAAGGCCCGAAGGACCATTTAGCCGCCAATATTTGTTCAAGCATTAAATATACTTGCC
TTGGCGCTGCGCATAGTTAACGATTTGCTAAGCGAGCCGCTGCGCGTGGGGAAATGTCCT
TGAGTCCCAAACAAACGACAACGACGCCATTTTCAGTAACGGATATCCTCAGCCCGCTCG
ACGAACCTTACGGCCAGCACGGTCCCGCGACGGTAGACGACGGCGGCAACCTGCAAACGC
GACGAGAAATTCACGACTCGTACAGCAGTGCGTCTATGGATCCAGGACATTCGGCAGTAG
CATCCAGTGGCTTGCACGTACCGAGCCTTGGCAGTTCTCTGAGCACCGGATCAACCTCGC
CTCCCATGTCGTCGCTTTACCGACAGCCGATGTCTATGGGCACGCCGCCGCACCACGGGG
TGCACCATCATCAGCAAATTCCGGCGGCGAGCGTACCTTATCAGAGTATGCCAACCGCGG
CGGCTGTTGCTGCTGGCATGAATTCCGGCTACAACCTCCACCCAATGGCACCTGCTCCTC
AGGCGACTTTTCACACGATGCCGGGATCAGGATCGTCAGGGTACTGCAACGGAACTGTAG
CCGACCTTCCGTCATA

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data