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CONSTRUCT

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  1. Clone 'ma218m13' > 
  2. Construct 'CONS00001450' > 
  3. Construct 'CONS00001473'

Construct

ID

CONS00001473

Name

p.Phmamm.REG.CG.MTP2014.S411:119129-121273>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in ???

This construct is testing Regulatory Region

REG00001451: Phmamm.REG.CG.MTP2014.S411:119129-121273|ELK

Sequence

Show sequence

Length: 2,145

AAGTGATTTCGACGCAAGGTTTAATGCATGTCTGTTTTCGGATGCGCATCCCTGCATGTA
AATGATGCGGCGCGATTTTTTACTAATCACATGATACAGAGACACCTCTGGTTGGTCACA
AACCCGCTTAACCTGACAAGAACCCGTTACTGCCTGACGAGCGGAAGTCGCTGTTAGCTT
TTGCACGCAATCCTGTACCAGGGAACAGGCAGTGTCTATTACTGTGGGAATCTACGTCTC
CCTGTGTCAAAACGCACGGCGTCTTCCGACAATTCTCCGTCTGTCATCGCGCCCTAAATA
ACGCCAACAAATCAAACACCTACCTGGGTCTTAATAACACGGGGCCTCTCACCTACGTGG
CCACAACAAGACTTGACAGACAGCCCCTAAATAAGGTCCTGTCTATGACCAGGTGACGAC
CAAGCATCACAGAGGTTAAGCTTCTGGTGCTACCTATTTTTAGACTTAGACCGCTGCAAT
ATGCCGGCTTGCATATATATATATATTGCTTGATAGCGCTTTCATTGGTCGAGTTGTTTG
TGGCACTTGTTGTGTCGTCAGCAGCGCCTACCTTTTTACGTCACGCCAGTGCTTCGGGCA
CTCCTGCGTGCACGCGAGACTTATTCATTCCTAAATAAGTAAATGGAATCTTCAGCGCCA
GGGGCAAAATCAAATTTCCATGTCCTCGGTTTTAGTGAGGCACGCTTCCGCTGCGCTTAA
ACCGTATTCTAAAGCTTAGGAGGAGCACTTGGCGTATTGAAACGTCTTATCGGAGTTTAA
TTACGACCGCAATGTTAGCGTGTTTCCCCTTTTCGTCGTTTCGTTTTCACCTAAATTCGC
TAATTTGGTGGTGAACTTTATTATTTATCACAGCTTTTTGGTTGTTAGAAGATTTTCTAA
AAAGAAAAATTATGCAAAACTGTTTGTTAGGTATTTTTTTGGTTTATCCCCCCCCCCTTA
CGACCGCAATGTTAGCGTGTTTCCCCTTTTCGTCGTTTCGTTTTCCCCTAAATTCGCGGG
TTTGTTGGTAAAGTTTAATACCAATACTTGTTCTGTTGTTTTTGAAAGATTTCCTAAAAA
GAAAAATTATGTAAAACTGTTTGTTAGGCCTTATGTTGTTTAAAGGAAACATTTCTGAAT
TCAAGTGCCTTTGTTTTGCATTGAGCATTATTAGCGTTTACAATTACAAACTGATATGTA
TCAAACACAAATGTATTATCACCATCATTAGCATAGAATTTTCGCAAACCGCACAATGCG
TTAGCCCGGACGATCCGGATTGGCGCTAAACAGCGATAACATTTCAGTAGCGCTTCCCGT
CATTCGCCTGTACTGTGTATTGTACCGGGGCTGTCGACGGATTGTGAAAAGTCGACGCGG
CCTGCCTTGATTTGACGGCTTGGAGCTGAACAGTGGCTTGGCAAATTTCCGCGCCCTTTC
TGCGAAAAAACACTACGCTCCCGCGAGCGCCTGCAATTGTCACAAACTCCGGGTTGAGGT
CCTGTGGAGCAAGCGGCAGCGTGGGCGGAAGGAAAACCGCCATTAATTAGGCGAGCACGC
CGCGCTCCAACCCTCATAATACTTTATGGTTGAAGTCTATTTGGAATGGTGAGCAGCGGC
AGCTTAACAGGATGCCGTCTGTCGGGGCCTTCGCTGGGATCACTCAGCCGCCTGTTCTAG
AAGCCTCGGGATCGCCTGACTGCAACGGTAGCGCCATCACAGAGCTGCAAGCGCGATTCC
CTTTCGGGCATGCCCGCCACTTCCCCGACGCCCCAACACCTCTTCCCGGTGTCGAATTCC
CGCCAGGAATCGTGCGTTGTAGCAGCTTGACATTATATAAGCTTGATAGCGTTGTGGCTG
CCAATCCGCAACTAGTGTTTAAACGCGCGACAGTTTTTATGCAATGCCGCGGTGCAATAT
GCCCCGTCTGGTAGTGATGAGCACATTTGAATTAAATAGGCAAAGTGTGTTTAATTTCAC
AATTAAATAGACAAAGTGTGTTTAATTTCACACAAACAATTACCAGGCCTATTGCATAAC
GTCTACTTACGTTAAAACCATAAAATGCTTTAGTTTGCCAAATCCTGGGACGGAAATAAA
ACGTTTTAGGGTTTGTTTTAAAATCTGCTTCGCAATAGGAGTGAG

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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