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CONSTRUCT

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  1. Construct 'CONS00001490'

Construct

ID

CONS00001490

Name

p.Phmamm.REG.CG.MTP2014.S14:106625-107597>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in endoderm.

This construct is testing Regulatory Region

REG00001468: Phmamm.REG.CG.MTP2014.S14:106625-107597|Tolloid

Sequence

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Length: 973

AAATAGACAGTCGGCTTGTGGTAACTTATTAAATATAAACGCGATTAAATTCCATCACAG
CATTTTCGCACCTGCTGAAATCTGAAACGAACTTAAATATCTATGATTGGAAGCGATAAG
GTAAACAAGCTGGGCATAATGCAGTCGTGCTTTTTTCTTCTCCTTTATCTGGCGCGCTTG
CATCGGCCCGCGACAGACTTGTTCGGTAATCAAACAACTTGGAGCTTCAAAAAGACGGTT
TGGTCTTGAGCGACTGCTTGGTGCGAAGTTATATGAGAAGGACAAACATCCAGCCGAAGT
CTTTTATGCCCCGCGTTTATATCGCTAGTCTTTCCTGTGGCGTAAATCAGTTTTAACTGA
AACACTGTACAGTCTACAGTGGACCAGTATTGTGCCAGTGTGTTATGTCCACGCAGAATT
AGAGGTTACATAGCTTGTTTTGTCCATTAGGTAAGAGGTTAGCACAGAGATAACGTGTCG
CAACACATAAGGTGTGGTTTAGTATGACATTCATGACACCAGTAAATATATGCGAGGAGT
ACACAAGAATTTGCAGTACGCTGTTCCGATAATGTCGTCCGTAACCTAGATTGTATCTCT
CAGTTTAGGTTGTTCGCTTTGCCTTCAGGGTTGTCACTCCAGTAAACGTTGTAAAAACTC
CTAGCTGATAAGATGGCATCAGATTTTGACATTTCCAGGACTTAAGCCACACTGTGTTGC
CACAATATATGAGTAATTTGTCAAGCCTTGTACATAGCGGTTTAGGGTTATCATTAAATC
CATCTTCAATGTTGCAGAGATTGCGCACAAACGTCCCCAAAAACGGGCATTACCACTTGA
TGCTACGCAGAAAAAAAATGTTGTTTTAAAATTGCTTGAGGCTGTAAAACATATTTTTTT
GTAAAAAAGATCGCACATTTGCAGCACTTACTTGCTGTAAACACTAAGTTCAGTTTATCA
AATTTGGATTGTT

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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